Description: A human uterine sarcoma derived in 1980 from a tumour obtained at the time of a hysterectomy from a 56 year old Caucasian female. The cells form tumours in athymic nude mice, readily form colonies in soft agar and have a reported doubling time of 22-24 hours. The cells are sensitive to a number of chemotherapeutic agents including doxorubicin, dactinomycin, mitomycin C, taxol and bleomycin. They are resistant to vinblastine dacarbazine, cisplatin, melphalan, vincristine methotrexate and etoposide. The multi drug-resistant cell line MES-SA/Dx5 was established from MES-SA cells grown in the presence of doxorubicin.

Also Known As:

Species: Human

Tissue: uterus

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: McCoy’s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:6 to 1:8 i.e. seeding at 1-4 x 10, 000 cells/cm² using 0.03% EDTA; 5% CO2; 37°C. Do not use trypsin.

Release Conditions: No

Depositor:

Originator: No

References: Cancer Res 1983; 43:4943

Hyperlink to ECACC Cell Line Data Sheet: 95051030