Description: Derived from fifteen repeated subcutaneous passages of mouse lung metastases, the parent line being TS/A.

Also Known As:

Species: Mouse

Tissue: lung

Growth Properties: Adherent

Morphology: Fibroblast

Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Release Conditions: No

Depositor:

Originator: No

References: J Nat Cancer Inst 1986;76:87

Hyperlink to ECACC Cell Line Data Sheet: 90062270