Description: ML-1 is one of three celll lines isolated in 1978 from the peripheral blood of a 24 year old male patient with acute myeloblastic leukaemia. The cells can convert to more mature cells by the use of DMSO. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Also Known As:

Species: Human

Tissue: blood

Growth Properties: Suspension

Morphology: Lymphoblastoid

Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Maintain cultures between 2-9×100,000 cells/ml; 5% CO2; 37°C. Freeze in 10% DMSO + 90% FBS. Immediately after resuscitation, pellet cells by centrifugation at 150 x g for 5 minutes and resuspend the cell pellet in fresh medium. This is to remove the presence of DMSO which may cause differentiation of the cells if allowed to remain.

Release Conditions: No

Depositor:

Originator: No

References: Ohyashiki K, Ohyashiki JH, Sandberg AA 1986 Cytogenetic characterization of putative human myeloblastic leukemia cell lines (ML-1,-2, and -3): origin of the cells. Cancer Res. 46(7):3642-7 PMID: 3458526.

Hyperlink to ECACC Cell Line Data Sheet: 88113007