Description: Melanoma cell line from lymph node

Description Key Words: Human melanoma

Also Known As: No other names

Organism: Human (Homo sapiens)

Tissue: Skin

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: RPMI 1640 (with 2mM L-Glutamine + 25mM Hepes) + 10% Foetal Calf Serum (FCS)

Resuscitation: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line.

Subculturing Procedure:

Medium Renewal:

Subcultivation Ratio: 1:4, Seeding density 0.8 x10⁴ cells/cm². Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min. MM138 has a relatively slow doubling time of around 60 hours. It does not become confluent and will detach after 6-7 days. If split too hard, the doubling time is greatly increased.

Culture Conditions: Cells incubated at 37°C with 5% CO2.

Cryoprotectant Medium: 10% DMSO + 90% FCS

Handling Procedure for Frozen Cells: Upon receipt, frozen ampoules should be transferred directly to liquid nitrogen storage without delay, if not to be used immediately. Storage at -80°C may result in loss of viability.

Additional Information:

Mutations:

– Homozygous deletion CDKN2A.

Depositor: Peter Parsons – Queensland Institute of Medical Research Australia

References:

R H Whitehead and J. H.Little

Tissue Culture Studies on Human Malignant Melanoma

Pigment Cell 1: 382-389 1973

Cellosaurus: CVCL_2602