MNNG/HOS (TE85″,” Clone F-5)
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Description
Description: A chemically transformed cell line (MNNG 0.01ug/ml) derived from an osteosarcoma of a 13 year female Caucasian. Grows to high saturation density and produces tumours in nude mice. Please note that frozen vials of this cell line are labelled: MNNG/ HOS.
Also Known As:
Species: Human
Tissue: bone
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:6 i.e. seeding at 2-4×10000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Nature 1975;256:751; Int J Cancer 1977;19:505
Hyperlink to ECACC Cell Line Data Sheet: 87070201
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