MOR/0.2R
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Description
Description: The drug resistant cell line MOR/0.2R has been derived from the parent line, MOR, by continuous exposure to increasing concentrations of doxorubicin (also known as adriamycin). MOR/0.2R accumulate lower levels of doxorubicin than the parent line and have been shown to overexpress multi drug resistance associated protein (MRP). Expression of a 190kDa membrane protein associated with the degree of drug-resistance has been indicated. Cells grow as easily detaching aggregates.
Also Known As:
Species: Human
Tissue: Lung
Growth Properties: Semi-adherent aggregates
Morphology:
Growth Medium: RPMI 1640 + 2mM Glutamine + 0.2µg/ml Doxorubicin + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split subconfluent cultures (70-80%) 1:2 to 1:4 seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. It is recommended to culture the cells without drug after resuscitation until the first passage. These cells produce a lot of cell debris.
Release Conditions: No
Depositor: Dr P Twentyman, UKCCCR, Lincolns Inn Fields, London
Originator: Yes
References: J Natl Cancer Inst 1994;86:110; Int J Cancer 1995;63:855
Hyperlink to ECACC Cell Line Data Sheet: 96042335
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