MT-4
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Description
Description: Derived from a 50 year old Japanese male with generalised lymphadenopathy and heptosplenomegaly (Adult T-cell Leukaemia) by co-culture of his peripheral leukocytes with male umbilical cord lymphocytes. The cells carry HTLV-1 and support the growth of HIV. The cells should be handled under laboratory containment level 3 conditions. STR profiling at ECACC confirms the presence of a Y chromosome
Also Known As:
Species: Human
Tissue: Lymphocyte
Growth Properties: Non-adherent or adherent on poly-L-lysine treated plastic
Morphology: Lymphoblast
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Decomplemented Foetal Bovine Serum (FBS).
Subculturing Procedure: Seed cultures between 2-3×100,000 cells/ml. Saturation density 9 x 100,000 cells/ml; 5% CO2; 37°C.
Release Conditions: Yes. Please complete attached form.
Depositor:
Originator: No
References: Harada et al., (1985) Infection of human T-lymphotropic virus type-1 (HTLV-1)-bearing MT-4 cells with HTLV-III (AIDS virus): chronological studies of early events. Virology, 146: 272-281.
Hyperlink to ECACC Cell Line Data Sheet: 08081402
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