NCI-H929
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Description
Description: This IgA-producing cell line has been established from a malignant effusion in a 62-year-old Caucasian woman with myeloma. They are positive for plasma cell antigen 1 (PCA-1)”, transferrin receptor and CD38 but negative for early B cell developmental markers. HLA DR and CALLA. NCI-H929 is negative for Epstein-Barr virus nuclear antigen. A rearrangement of the c-myc proto-oncogene has been observed as well as the expression of c-myc RNA and an activated ras allele.
Also Known As:
Species: Human
Tissue: unknown
Growth Properties: Suspension
Morphology: Lymphoblastoid
Growth Medium: RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Maintain cultures between 2-4 x 100, 000 cells/ml; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Blood 1986;67:1542; ibid 1988;72:1163; Mol Cell Biol 1988;8:124
Hyperlink to ECACC Cell Line Data Sheet: 95050415
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