NCTC 4206
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Description
Description: A chemically defined medium derivative of B14FAF28-G3. Since it was established, NCTC cells have been employed in nutritional studies involving 6 different NCTC chemically defined serum-free media.
Also Known As:
Species: Hamster
Tissue: peritoneum
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: NCTC 135 100% + 2mM Glutamine.
Subculturing Procedure: Sub-cultures are prepared by scraping; 5% CO2; 37°C. Seed cells between 2-4x10e4/cm using the higher seeding level upon resuscitation. Cells can take up to 10 days after resuscitation before being ready to sub culture. Cells routinely take up to a week to reach a level suitable for subculture. Media change every 3-4 days. Freeze in 45% Fresh media, 45% Conditioned media and 10% DMSO.
Release Conditions: No
Depositor: Obtained from ATCC
Originator: No
References: Exp Cell Res 1964;36:439
Hyperlink to ECACC Cell Line Data Sheet: 91010303
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