NCTC clone 1469
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Description
Description: Derived from mouse liver cell line NCTC 721 which was obtained from normal liver of a 2 day old C3H/An mouse. The cells have been used in biochemical and nutritional studies. The cells are susceptible to VSV (Indiana strain).
Also Known As:
Species: Mouse
Tissue: Liver
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: NCTC 135 + 2mM Glutamine + 10% Horse Serum (HS)
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:4 using only mechanical techniques, such as scraper, do not use trypsin, 5-10% CO2; 37°C. Change media every 3 days. Freeze in 10% glycerol/90% FBS. Cells are shed into the medium giving it a turbid appearance similar to bacterial contamination. Suspension cells are viable and can be used for further subculture.
Release Conditions: No
Depositor:
Originator: No
References: J Nat Cancer Inst 1952;12:1245; J Nat Cancer Inst 1957;18:701; J Nat Cancer Inst 1959;23:823
Hyperlink to ECACC Cell Line Data Sheet: 88111403
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