ND-C
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Description
Description: ND-C hybrid cells were derived after fusion of primary neonatal-rat dorsal-root-gauglion (DRG) neurons with N18Tg2, a mouse neuroblastoma (C1300) derived azaguanine resistant line. HAT sensitive hybrids were selected and cloned by limiting dilution. The cells express rat Thy 1.1 marker and the sensory neuropeptide substance P. The cell line has been mycoplasma eradicated at ECACC.
Also Known As:
Species: Mouse
Tissue: Neural
Growth Properties: Adherent
Morphology: Neuronal
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3 x 10,000 cells/cm²; aspirate or gently tap cells loose, use of trypsin not necessary; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr J N Wood, The Sandoz Institute for Medical Research, London
Originator: Yes
References: Proc R Soc (B) 1990;241:187
Hyperlink to ECACC Cell Line Data Sheet: 92090913
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