Description: A mouse embryonic stem cell (ES) line (Nd) containing a novel fluorescent reporter (VNP) to investigate the temporal dynamics of Nanog expression in mouse and to dissect the lineage potential at different Nanog expression levels. This novel reporter ES cell line allows the accurate monitoring the dynamic expression of the transcription factor Nanog in the pluripotent state. The cell line will be a valuable tool to obtain quantitative measurements of global gene expression in pluripotent mouse ES cells in different states, allowing a detailed molecular mapping of the pluripotent state.

Also Known As:

Species: Mouse

Tissue: Embryo

Growth Properties: Adherent

Morphology: Small round cell growing in spherical colonies

Growth Medium: Culture cells in Glasgow Modified Eagles Medium with 10% FCS + 2mM L-glutamine, 2ng/ml Leukemia Inhibitory Factor (LIF) and 1mMolar 2-mercaptoethanol on gelatin-coated (0.1%) cell culture plastic.

Subculturing Procedure: Cultures must be incubated in a humidified 10% CO₂/95% air incubator at 37^?C. Subculture with 0.05% Trypsin/EDTA and inoculate new cultures at 3 x 10⁴ per cm².

Release Conditions:  No

Depositor: Ana MV Guedes

Originator: Yes

References: Abranches E, Bekman E, Henrique D. Generation and characterization of a novel mouse embryonic stem cell line with a dynamic reporter of Nanog expression. PLoS One. 2013; 8(3):e59928. doi: 10.1371/journal.pone.0059928. Epub 2013 Mar 19. Erratum in: PLoS One. 2014;9 (6):e101028. PubMed PMID: 23527287; PubMed Central

Hyperlink to ECACC Cell Line Data Sheet: 16010601