ND8/34
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Description
Description: The hybrid ND8/34 is a subclone of ND8 which was derived after fusion of primary neonatal rat dorsal-root-ganglion (DRG) neurons with N18Tg2, a mouse neuroblastoma (C1300)-derived azaguanine-resistant line. HAT sensitive hybrids were selected and cloned by limiting dilution. ND8/34 are electrically excitable to the chemical activator of nociceptive sensory neurons, bradykinin.
Also Known As:
Species: Rat/mouse hybrid
Tissue: neural
Growth Properties: Adherent
Morphology: Neuronal
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3×10,000 cells/cm² by either aspirating or gently tapping the cells loose; 5% CO2; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.
Release Conditions: No
Depositor: Dr J N Wood, Neuroimmunology Dept, The Sandoz Institute, London
Originator: Yes
References: Proc Royal Soc (B) 1990;241:187
Hyperlink to ECACC Cell Line Data Sheet: 92090904
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