Neuro 2a
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Description
Description: Derived from a spontaneous tumour in an albino strain A mouse. Cells produce microtubular protein which is believed to play a role in the contractile system giving axoplasmic flow in nerve cells.
Also Known As:
Species: Mouse
Tissue: nerve
Growth Properties: Adherent
Morphology: Neuronal/amoeboid-like
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 4×10,000 cells cm²; 5% CO2; 37°C. After resuscitation cells can take up to 6 days before a monolayer forms. These cells only adhere to the culture flask very lightly, care should be taken when subculturing. There is no need to wash the cells with PBS or trypsin, merely pour off half of the spent medium carefully, give the flask a gentle knock and resuspend the cells in fresh medium using a pipette to gently disperse any clumps of cells to finally reseed at 1:2. On arrival, growing cultures should be spun out and re-seeded in fresh media.
Release Conditions: No
Depositor:
Originator: No
References: J Cell Biol 1969;43:69A; Proc Natl Acad Sci, USA 1970;65:129
Hyperlink to ECACC Cell Line Data Sheet: 89121404
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