NIH 3T3
[Create a profile and sign in to view pricing and add to cart]
- Description
- Ask a Question
Description
Description: Established from a NIH Swiss mouse embryo. These cells are sensitive to sarcoma virus focus formation and leukemia virus propogation. Cells have now lost their contact inhibition.
Also Known As:
Species: Mouse
Tissue: Embryo
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: DMEM + 2mM Glutamine + 10% Calf Serum (CS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5×10000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C. Do not allow culture to become fully confluent; the use of fetal calf serum is not recommended.
Release Conditions: No
Depositor:
Originator: No
References: J Virol 1969;4:549; Cell 1979;16:63; Cell 1979;16:347
Hyperlink to ECACC Cell Line Data Sheet: 93061524
Ask a Question
Have a question about this product? Our technical team is here to help.
