NS0
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Description
Description: NS0 is a non-Ig secreting, non-light chain-synthesising subclone of NS-1 (P3/NS1/1-Ag4-1 ECACC catalogue no. 85011427). The cells are resistant to 10µM azaguanine, cells die in the presence of HAT medium. The NS0 cell line is the property of the Medical Research Council (MRC). ECACC is unable to supply this cell line to commercial entities unless a license has been first secured from MRC Technology. Requests for commercial use of this cell line will be forwarded to MRC Technology.
Also Known As:
Species: Mouse
Tissue: myeloma
Growth Properties: Suspension
Morphology: Lymphoblast
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Maintain cultures between 3-9×100,000 cells/ml; 5% CO2; 37°C. Cells can adhere loosely to the culture vessel surface; remove by tapping against the flask. Cultures that are more than 2 days old may have to be scraped.
Release Conditions: Yes – commercial organisations are required to complete the ‘Cell Line Release Authorisation for Research Use in Commercial Organisations’ release conditions form.
Depositor: Dr J Jarvis, MRC Laboratory of Molecular Biology, Cambridge
Originator: Yes
References: Methods in Enzymology 1981;73B:3
Hyperlink to ECACC Cell Line Data Sheet: 85110503
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