NS20Y-BU-2
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Description
Description: A bromodeoxyuridine-resistant mutant of NS20Y (ECACC catalogue number 08062517) The cell line NS20Y is a cholinergic cell line derived from mouse neuroblastoma C1300 (Amano et al.”, (1972) Neurotransmitter synthesis by neuroblastoma clones. Proc. Natl. Acad. Sci. 69: 258-262) Mouse strain A/Jax.
Also Known As:
Species: Mouse
Tissue: Neuroblastoma
Growth Properties: Adherent
Morphology: Neuronal
Growth Medium: DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS)+ 1 mM 8-bromodeoxyuridine. Cells can also be grown in the absence of bromodeoxyuridine
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: no
Depositor: Professor Bernd Hamprecht, Interfaculty Institute for Biochemistry, University of Tuebingen, Hoppe-Seyler Street 4, 72076 Tuebingen, Germany.
Originator: Yes
References: Heumann et al.,” (1979) Clonal hybrid cell lines expressing cholinergic and adrenergic properties. Proc. Natl. Acad. Sci. 76: 4674-4677.
Hyperlink to ECACC Cell Line Data Sheet: 08062502
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