Description: The pluripotent human embryonal carcinoma cell line NTERA-2 clone D1, also known as NT2/D1, is a subclone derived from the parent line NTERA-2. NTERA-2 cells were established from a nude mouse xenograft tumour of TERA-2 cells, which were originally isolated from a lung metastasis from a 22 year old patient with primary embryonal carcinoma of the testis. The subline NTERA-2 clone D1 differentiates into neuronal and other cell types in response to retinoic acid (RA) or hexamethylene bisacetamide (HMBA). Differentiated cells become permissive for human cytomegalovirus (HCMV) or human immunodeficiency virus (HIV). Differentiation with RA as inducer results in glycolipid changes, appearance of neurons and induction of Homeobox (HOX) gene clusters. When NTERA-2 clone D1 cells are passaged at high cell density EC-like cells predominate. However, when cultured at low density many large flat cells differing from typical EC cells are formed.

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Species: Human

Tissue: Testis (lung metastasis)

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: DMEM containing 4.5 g/L glucose + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Cells should be cryopreserved in 95% FCS:5% DMSO

Subculturing Procedure: Split confluent cultures (approximately 30 x 10,000 cells/ cm²) by gentle scraping to dislodge cells from the flask surface. Cell sheets/clumps can be dispersed by gently pipetting. Cultures should be maintained at high density by seeding new flasks at a density of at least 6 x 10,000 cells/ cm². Medium should be renewed every 2-3 days and subcultured at approximately 5 days. As the embryonal cells tend to grow in large clumps and must be removed by gentle scraping , viable counts of only 40-50% are normal on resuscitation and when sub-culturing.

Release Conditions: No

Depositor:

Originator: No

References: Andrews PW, Damjanov I, Simon D, Banting GS, Carlin C, Dracopoli NC, Fogh J. 1984 Pluripotent embryonal carcinoma clones derived from the human teratocarcinoma cell line Tera-2. Differentiation in vivo and in vitro. Lab Invest. 50(2):147-62. PMID: 6694356

Hyperlink to ECACC Cell Line Data Sheet: 01071221