Nthy-ori 3-1
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Description
Description: Normal human primary thyroid follicular epithelial cells were transfected with a plasmid containing an origin-defective SV40 genome (SV-ori) to immortalise them. SV40 DNA was able to immortalise normal thyroid epithelial cells with the retention of several different functions.
Also Known As:
Species: Human
Tissue: Thyroid
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr D Wynford-Thomas, Department of Pathology, University of Wales, College of Medicine
Originator: Yes
References: Lemoine NR, Mayall ES, Jones T, Sheer D, McDermid S, Kendall-Taylor P, Wynford-Thomas D 1989 Characterisation of human thyroid epithelial cells immortalised in vitro by simian virus 40 DNA transfection. Br J Cancer. 60(6):897-903 PMID: 2557880.
Hyperlink to ECACC Cell Line Data Sheet: 90011609
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