Description: OACM5.1 C was established from a lymph node metastasis derived from a. primary adenocarcinoma of distal esophagus with the presence of Barretts transformed mucosa. The patient was a 47 year-old Caucasian female and exhibited lymph node involvement and distant pleural metastases. OACM5.1 C was found to be tumourigenic in NMRI (female nude mice or NOD-SCID mice.

Cultures derived from ECACC stocks of this cell line have been whole genome sequenced (Contino et al 2016) confirming the presence of many of the known mutations that drive oesophageal cancer.

Also Known As:

Species: Human

Tissue: Oesophagus

Growth Properties: Adherent

Morphology: Multicellular floating spheroids some with fibroblastoid morphology

Growth Medium: RPMI-1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 3 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Release Conditions: No

Depositor: Dr W.N.M. Dinjens, Department of Pathology, Josephine Nefkens Institute, Erasmus MC, University Medical Centre, Rotterdam PO Box 2040, 3000CA Rotterdam

Originator: Yes

References: Boonstra JJ, van Marion R, Beer DG, Lin L, Chaves P, Ribeiro C, Pereira AD, Roque L, Darnton SJ, Altorki NK, Schrump DS, Klimstra DS, Tang LH, Eshleman JR, Alvarez H, Shimada Y, van Dekken H, Tilanus HW, Dinjens WN. 2010 Verification and unmasking of widely used human esophageal adenocarcinoma cell lines. J Natl Cancer Inst. 102(4):271-4.PMID: 20075370.

 Whole-genome sequencing of nine esophageal adenocarcinoma cell lines

Hyperlink to ECACC Cell Line Data Sheet: 11012006