OAW42
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Description
Description: From an ascites of patient with ovarian cystadenocarcinoma. Has retained ability to form free floating cysts in vitro and produces extracellular matrix. Has defined chemosensitivity pattern. Valuable for studies on biology of human ovarian cancer.
Also Known As:
Species: Human
Tissue: ovary
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 20 IU/l Bovine Insulin + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr A Wilson, Oncology Research Laboratory, Derby City Hospital
Originator: Yes
References: J Nat Cancer Inst 1984;72:513; Hills CA, Kelland LR, Abel G, Siracky J, Wilson AP, Harrap KR 1989 Biological properties of ten human ovarian carcinoma cell lines: calibration in vitro against four platinum complexes. Br J Cancer. 59(4):527-34 PMID: 2653399.
Hyperlink to ECACC Cell Line Data Sheet: 85073102
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