P3X63Ag8

CODE: 85011401
Mouse myeloma

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Description

Description: Derived from the P3X27 cell line, itself a derivative of mineral oil-induced plasmacytoma MOPC-21 in BALB/c mice. Resistant to 20µg/ml azaguanine, does not grow in selective medium. HGPRT neg., dies in HAT. Doubling time 16-26 h.

Also Known As:

Species: Mouse

Tissue: skin

Growth Properties: Suspension

Morphology: Lymphoblast

Growth Medium: DMEM or RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Maintain cultures between 5×10,000 to 3×100,000 cells/ml; 5% CO2; 37°C. Cells will achieve maximum cell density of 5×100, 000 cells/ml.

Release Conditions: No

Depositor:

Originator: No

References: Exp Cell Res 1970;60:61; Eur J Immunol 1976;6:511

Hyperlink to ECACC Cell Line Data Sheet: 85011401

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