P3X63Ag8
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Description
Description: Derived from the P3X27 cell line, itself a derivative of mineral oil-induced plasmacytoma MOPC-21 in BALB/c mice. Resistant to 20µg/ml azaguanine, does not grow in selective medium. HGPRT neg., dies in HAT. Doubling time 16-26 h.
Also Known As:
Species: Mouse
Tissue: skin
Growth Properties: Suspension
Morphology: Lymphoblast
Growth Medium: DMEM or RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Maintain cultures between 5×10,000 to 3×100,000 cells/ml; 5% CO2; 37°C. Cells will achieve maximum cell density of 5×100, 000 cells/ml.
Release Conditions: No
Depositor:
Originator: No
References: Exp Cell Res 1970;60:61; Eur J Immunol 1976;6:511
Hyperlink to ECACC Cell Line Data Sheet: 85011401
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