Description: Established from a grade 4 prostatic adenocarcinoma from a 62 year old male Caucasian. The cells grow in agar and produce tumours in nude mice. Exhibit low acid phosphatase and testosterone-5-alpha reductase activity. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Also Known As:

Species: Human

Tissue: prostate

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: Coons Modified Hams F12 + 2mM Glutamine + 7% Foetal Bovine Serum (FBS). OR Kaigns modified Hams F12 + 45mg/L ascorbic acid + 18 mg/L Inositol + 2mM Glutamine + 7% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5×10000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C. Medium change every 5 days. The initial subculture interval after cells are thawed may be longer than 7-9 days.

Release Conditions: No

Depositor:

Originator: No

References: Invest Urology 1979;17:16; Cancer Res 1980;40:524

Hyperlink to ECACC Cell Line Data Sheet: 90112714