PG13
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Description
Description: PG13 cells were derived from TK-NIH/3T3 cells. This retrovirus packaging cell line is based on the gibbon ape Leukaemia Virus (GaLV). Introduction of retroviral vectors results in the production of retrovirus virions capable of infecting cells from many species excluding mice. Introduced DNA may be lost after long-term passage. Selection against loss can be performed by growing the cells in medium with dialysed FBS and100nM amethopterin for 5 days followed by cultivation in HAT and untreated FBS for 5 days. Maintain cells for 4 days in HT medium to avoid toxic effects of residual amethopterin. Virus production has not been observed, but there is the risk that cells produce a virus similar to GaLV. The cell line has been tested positive for Reverse Transcriptase.
Also Known As:
Species: Mouse
Tissue: Embryo
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: J Virol 1991;65:2220; Biotechniques 1989;7:980
Hyperlink to ECACC Cell Line Data Sheet: 95110215
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