PLC/PRF/5
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Description
Description: Alexander cell line which produces Hepatitis B antigen. The virus genome may be inducible in selective media. The cells should be handled under laboratory containment level 2. Expresses c-abl, c-fes, c-fms, c-myc, c-ha-ras and c-sis oncogenes. However there is currently no evidence that this cell line produces infectious Hepatitis B virus.
Also Known As:
Species: Human
Tissue: liver
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Proc Natl Acad Sci, USA 1985;82:83; J Virol 1979;32:796; Science 1980;209:497; Science 1983;222:385; Nature 1979;282:615
Hyperlink to ECACC Cell Line Data Sheet: 85061113
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