PNT2
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Description
Description: Established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from a prostate of a 33 year old male at post mortem. PNT2 cells contain the SV40 genome and express large T protein. They possess a well differentiated morphology with the expression of cytokeratin 8,18 and 19 with the latter being a feature of differentiated luminal cells of the glandular prostate. Cytokeratin 14, a marker of epithelial basal cells, is not expressed. The cells are non-tumourigenic in nude mice.
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Species: Human
Tissue: prostate
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: RPMI 1640 + 2mM Glutamine + 6 -10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr P Berthon, Dr O Cussenot, Hôpital SAINT-LOUIS, Paris
Originator: Yes
References: J Urol 1991;146(3) 881-6; Int J Oncol 1995;6; Int J Cancer 1995;62:724, Biochem Pharm 1999;58: 279-284
Hyperlink to ECACC Cell Line Data Sheet: 95012613
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