PSA1
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Description
Description: Thioguanine and ouabain resistant derivative of SIM. When cultured in absence of STO feeder cells it is stimulated to develop cell aggregates which differentiate resulting in formation of layer of endodermal cells covering aggregates.
Also Known As:
Species: Mouse
Tissue: embryo
Growth Properties: Suspension
Morphology: Teratocarcinoma
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Maintain cultures between 3-9×100,000 cells/ml; 5% CO2; 37°C. Requires STO feeder layer.
Release Conditions: No
Depositor:
Originator: No
References: Proc Natl Acad Sci, USA 1975;72:1441; Macpherson I,1973, Tissue Culture Methods and applications – Academic Press NY, 241; Dev Biol 1977;61:230
Hyperlink to ECACC Cell Line Data Sheet: 85011403
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