R2C
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Description
Description: A rat testis Leydig cell tumour cell line derived from a 2-month-old male WFu rat. Virus susceptibility: herpes simplex; vaccinia; pseudorabies; vesicular stomatitis (Indiana). Resistant to poliovirus 2. Stimulation by other hormones or cAMP is not required for hormone secretion.
Also Known As:
Species: Rat
Tissue: Testis
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: HAMS F10 + 2mM Glutamine + 15% Horse serum + 2.5% Foetal Bovine Serum (FBS). Do not use heat-inactivated serum.
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5×10,000 cells/cm² using 0.25% trypsin/EDTA;5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Shin SI et al.,” (1968) Studies on interstitial cells in tissue culture. II. Steroid biosynthesis by a clonal line of rat testicular interstitial cells. Endocrinology 82: 614-616.
Hyperlink to ECACC Cell Line Data Sheet: 89031606
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