Description: Established in 1963 from Burkitt's lymphoma in an 11 year old male. Growth is in the form of single cells without attachment and as macroscopically visible clumps containing many hundreds of cells. Resistant to VSV. This cell line carries the latent Epstein-Barr Virus (EBV) genome and is positive for EBNA. RAJI is sometimes referred to as a 'non-producer'; the EBV genome carries deletions attributed to preventing the formation of virus particles. Ethnicity: Black.

Also Known As:

Species: Human

Tissue: lymph

Growth Properties: Suspension

Morphology: Lymphoblastoid

Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: If starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube, and then slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100μl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 – 150 x g for a maximum of 5 minutes. Remove medium and re-suspend the cell pellet at a density of approximately 5 x 100,000 cells/ml into fresh medium containing 20% serum. Incubate flask at 37°C; 5 – 7% CO2. Check daily and keep flask in a vertical position until the cells reach the exponential phase of growth. This can take up 3-4 days. Once the culture is established the serum concentration can be reduced to 10%. Maintain cultures between 3-9×100, 000 cells/ml; 5% CO2; 37°C.

Release Conditions: No

Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford

Originator: No

References: Pulvertaft JV 1964 CYTOLOGY OF BURKITT'S TUMOUR (AFRICAN LYMPHOMA).Lancet. 1:238-40 PMID: 14086209; J Nat Cancer Inst 1966;37:547; Trans NY Acad Sci 1966;29:61

Hyperlink to ECACC Cell Line Data Sheet: 85011429

Hyperlink to Wikipedia page:   Raji