RIN-5F
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Description
Description: RIN-5F is a secondary clone of the rat islet tumour cell line RIN-m (ECACC catalogue no. 95071701). Cloning was performed by limited dilution in RPMI 1640, 10%FBS and 20% conditioned medium. The conditioned medium contained growth hormones and prolactin being produced by rat pituitary tumour cells GH3 (ECACC catalogue no. 87012603). RIN-5F cells secrete insulin, produce high levels of the key APUD enzyme, L-dopa-decarboxylase and contain membrane-bound secretory granules.
Also Known As:
Species: Rat
Tissue: Pancreas
Growth Properties: Adherent
Morphology:
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells grow in small islands and do not form a confluent monolayer.
Release Conditions: No
Depositor:
Originator: No
References: Proc Natl Acad Sci USA 1977;74:628; ibid 1980;77:3519; Diabetes 1982;31:521; Endocrinology 1983;112:1070; Proc Soc Exp Biol Med 1983;175:35
Hyperlink to ECACC Cell Line Data Sheet: 95090402
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