SBAC
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Description
Description: Derived from the fasciculata-reticularis zone of the adrenal cortex taken from three normal adult cows. FGF stimulates cell division. Cells are responsive to ACTH and other steroidogenic agents. Finite lifespan; 60-70 population doublings expected. Studies have indicated that this cell line produces both detectable BVDV antigens and infectious BVDV.
Also Known As:
Species: Bovine
Tissue: adrenal cortex
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: Ham’s F12 with Fibroblast Growth Factor (FGF) (crude, 50ng/ml or pure, 500 pg/ml) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: In Vitro 1987;23:247; Endocrinology 1982;111:919; J. Virol. Methods 1994;24:211
Hyperlink to ECACC Cell Line Data Sheet: 91021201
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