SCC-4
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Description
Description: Derived from a human squamous cell carcinoma (SCC) of the tongue from a 55-year-old male. SCC-4 cells have been reported to form colonies in semi-solid medium, and are not induced to differentiate by anchorage deprivation. Growth is enhanced by use of a feeder layer of 3T3 swiss cells.
Also Known As:
Species: Human
Tissue: Tongue
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 ug/ml hydrocortisone
Subculturing Procedure: Split confluent cultures 1:3 to 1:4 i.e. seeding at 1-4 x10, 000 cells/cm² using trypsin/EDTA; 5% CO2; 37°C
Release Conditions: No
Depositor:
Originator: No
References: Rheinwald & Beckett (1981). Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultured from human squamous cell carcinomas. Cancer Res 41: 1657-1663
Hyperlink to ECACC Cell Line Data Sheet: 89062002
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