TR33B
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Description
Description: Ethyl nitrosourea induced tumour of the spinal cord and roots from a Wistar-Furth rat. In cell culture the cells grow to a high density and form a sheet of cells with many processes. TR33B is the parent line of 33B (ECACC catalogue no. 85081901).
Also Known As:
Species: Rat
Tissue: Brain
Growth Properties: Adherent
Morphology: Glial
Growth Medium: DMEM + 2mM Glutamine + 10% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr K Fields, Albert Einstein College of Medicine, Bronx, New York
Originator: Yes
References: Proc Natl Acad Sci, USA 1975;72:1296
Hyperlink to ECACC Cell Line Data Sheet: 85040102
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