tsA201
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Description
Description: tsA201 is a transformed human kidney (293, ECACC catalogue no. 85120602) cell line stably expressing an SV40 temperature-sensitive T antigen. The cell line has been used in a variety of functional expression assays and has been reported to produce high levels of recombinant proteins. Transfection efficiency decreases over a period of several months, therefore it is advisable to lay down a frozen stock as a reserve. The cells have been eradicated from mycoplasma at ECACC. The identity of tsA201 and 293 has been confirmed by STR profiling.
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Species: Human
Tissue: Kidney
Growth Properties: Adherent
Morphology: Epithelial-like
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:8; i.e. seeding at 2-4×10, 000 cells/cm² using mechanical agitation or EDTA solution; 5% CO2; 37°C. Do not let cells become over confluent as this can affect morphology.
Release Conditions: No
Depositor:
Originator: No
References: J Membrane Biol 1996;152:39; Gene 1995;156:235; PNAS USA 1994;91:12785; Pflügers Arch 1994;427:136; J Gen Physiol 1994;104:507; BioTechniques 1993;15:906
Hyperlink to ECACC Cell Line Data Sheet: 96121229
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