V79
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Description
Description: Established after spontaneous transformation of cells isolated from the lung of a normal Chinese hamster (male). The G1 phase in these cells is either absent or very short. Consequently they have been used in studies of the G1 growth period. A clone called V79.4 is available (ECACC catalogue no. 93010723). V79 is well established in toxicology studies. Stability of karyotype and morphology is making them suitable for gene toxicity assays with low background aberrations. The cells do not express endogenous cytochrome P450 and have been used for the insertion and expression of P450 from human, rat, mouse and fish.
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Species: Hamster
Tissue: lung
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:12 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr D Wynford-Thomas, Department of Pathology, University of Wales, College of Medicine
Originator: Yes
References: J Cell Biol 1967;34:684; Proc Natl Acad Sci, USA 1978;75:2873; Somatic Cell Gen 1979;95:615
Hyperlink to ECACC Cell Line Data Sheet: 86041102
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