Vero (AC-free)
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Description
Description: Vero cell line (ECACC catalogue no. 84113001) adapted to grow in animal component free medium (VP-SFM (Gibco cat no. 11681-020)).
Also Known As:
Species: Monkey
Tissue: Kidney
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: VP-SFM (Gibco cat no. 11681-020) + 4mM Glutamine. When freezing cells down use culture medium (50:50 conditioned medium:fresh medium) + 10% DMSO.
Subculturing Procedure: In order to prevent syncitia formation, do not allow cells to become confluent. Split semi-confluent cultures 1:3 i.e. seeding at 4×10,000 cells/cm² using an animal component free dissociation reagent; incubate at 37°C. Please note: Once cells have detached from the flask it is necessary to neutralise the dissociation reagent using a compatible inhibitor . Centrifuge the cells; 100-150 x g for 3-5 minutes; count and re-suspend at the recommended seeding density in fresh medium
Release Conditions: Yes – CITES (Convention on the International Trade in Endangered Species of Wild Fauna and Flora) licence required for all orders outside the European Union
Depositor: Dr. E. Penn, European Collection of Cell Cultures, Health Protection Agency, Porton Down, Salisbury,”
Originator: Yes
References: None Specified By Depositor
Hyperlink to ECACC Cell Line Data Sheet: 08011101
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