Description: A World Health Organisation working cell bank of 1,000 ampoules deposited at ECACC, derived from an original American Type Culture Collection (ATCC) ampoule. Available free of charge to organisations producing vaccines following receipt of approval to supply from WHO.

Criteria for acceptability of request: production of a vaccine or biological defined in WHO recommendations/guidelines; development of new candidate vaccines or biologicals of global health importance as determined by WHO; exceptionally, samples may be released for reverse genetics or similar vector production intended for manufacture or development of vaccines or biologicals.

Contraindications: transferring the released cells and their progenies to third parties without permission of WHO; use for testing or cell therapy applications.

A Certificate of Analysis is not available for this cell line. The WHO Vero reference cell bank 10-87 cells are not "approved" in any way for vaccine manufacture by WHO. These repository cells are provided for the purpose of establishing master cell banks (MCB) and master working cell banks (MWCB). Regardless of any previous testing, any cells transferred to, and cultured in, a new environment will require re-qualification. Therefore, it is the responsibility of each manufacturer to ensure appropriate handling, expansion, and maintenance of the cells and to perform the qualification tests necessary to establish a cell bank for vaccine manufacture.

Also Known As:

Species: Chlorocebus sabaeus

Tissue: Kidney

Growth Properties: Adherent

Morphology: Fibroblast-like

Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: A full description of the recommended subculture methods and documentation of the cell bank production is provided with the cells.

Release Conditions: Yes – (1) CITES (Convention on the International Trade in Endangered Species of Wild Fauna and Flora) licence required for all orders outside the European Union and (2) completion of the attached Agreement form (M021 Vero WHO Deposit Release Form)

Depositor: World Health Organisation Geneva

Originator: No

References: Osada, Naoki, Arihiro Kohara, Toshiyuki Yamaji, Noriko Hirayama, Fumio Kasai, Tsuyoshi Sekizuka, Makoto Kuroda, and Kentaro Hanada. "The Genome Landscape of the African Green Monkey Kidney-derived Vero Cell Line." DNA Research : An International Journal for Rapid Publication of Reports on Genes and Genomes 21, no. 6 (2014): 673-83

Hyperlink to ECACC Cell Line Data Sheet: 88020401