WERI
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Description
Description: Human retinoblastoma cell line derived from a female Caucasian. The cells are semi-adherent, some neuronal differentiation can occur when subculturing. Tumourigenic in rabbits.
Also Known As:
Species: Human
Tissue: Retina
Growth Properties: Semi-adherent
Morphology: Grape like clusters of round cells
Growth Medium: Iscove's Modified DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS) + 10ug/ml Insulin + 55uM 2-Mercaptoethanol
Subculturing Procedure: Split subconfluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-3 x100,000 cells/ml; 5% CO2; 37°C. Saturation density: 2 x 1000,000 cells/ml. Cells are semi-adherent. Scrape cells from surface; do not use trypsin.
Release Conditions: No
Depositor:
Originator: No
References: Corson et al.,” (2005) KIF14 is a candidate oncogene in the 1q minimal region of genomic gain in multiple cancers. Oncogene 14; 24 (30): 4741-54
Hyperlink to ECACC Cell Line Data Sheet: 06070602
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