XrS6
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Description
Description: Derived from the parent line CHO-K1 and is hypersensitive to ionising radiation but not to UV irradiation. Cells may revert to wild-type after 1-2 weeks in culture. They should be checked for sensitivity and recloned when necessary.
Also Known As:
Species: Hamster
Tissue: ovary
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2×10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr P Jeggo, MRC Cell Mutation Unit, University of Sussex, Brighton
Originator: Yes
References: None specified by depositor
Hyperlink to ECACC Cell Line Data Sheet: 92052128
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