Description: Rat hybridoma producing antibody against mouse Thy-1 (CD90). Cambridge Enterprise Reference number AB11:www.enterprise.cam.ac.uk.YBM 29.2.1 has been used in flow cytometry and in vitro T cell depletion studies.

Also Known As:

Species: Rat

Growth Medium: Iscoves Modified Dulbeccos Medium (IMDM) + 2mM glutamine + 5% Foetal Bovine Serum (FBS). Where the cells are grown under static conditions, 0.02% EDTA, trypsin or mechanical agitation maybe required to dislodge them from the plastic.

Subculturing Procedure: Maintain cultures between 3-9 x 100,000 cells/ml. 5% CO2; 37°C. Saturation density 9 x 100,000/ml

Release Conditions: Yes

Depositor: Cambridge Enterprise Limited, Hauser Forum, 3 Charles Babbage Road, Cambridge CB3 0GT. Originators: Professor H Waldmann and Dr SP Cobbold when at the Department of Pathology, University of Cambridge

Originator: Yes

References: Hale G, Clark M, Waldmann H. 1985 Therapeutic potential of rat monoclonal antibodies: isotype specificity of antibody-dependent cell-mediated cytotoxicity with human lymphocytes. J Immunol. 134(5):3056-61 PMID: 3980990. Cobbold S, Waldmann H.1986 Skin allograft rejection by L3/T4+ and Lyt-2+ T cell subsets.Transplantation. 41(5):634-9 PMID: 3085299. Erratum in Transplantation 1986 42(3):332.

Hyperlink to ECACC Cell Line Data Sheet: 11060806