Description: The antibody is directed against the monomorphic determinant of the mouse Thy-1 T cell antigen. Has been used in T+NK cell depletion in vivo and flow cytometry studies. Cambridge Enterprise Reference number AB10:www.enterprise.cam.ac.uk.

Also Known As:

Species: Rat

Growth Medium: RPMI 1640 + 2mM glutamine + 0.05mM 2-mercaptoethanol (2ME) + 20% Foetal Bovine Serum (FBS). These cells may adhere very firmly to standard cell culture flasks therefore ECACC suggests using ultra low attachment flasks (e.g. Corning 3814).

Subculturing Procedure: Maintain cultures between 3-9 x 100,000 cells/ml. 5% CO2; 37°C. Saturation density 9 x 100,000/ml

Release Conditions: Yes

Depositor: Cambridge Enterprise Limited, Hauser Forum, 3 Charles Babbage Road, Cambridge CB3 0GT. Originators: Professor H Waldmann and Dr SP Cobbold when at the Department of Pathology, University of Cambridge

Originator: Yes

References: Cobbold SP, Jayasuriya A, Nash A, Prospero TD, Waldmann H. 1984 Therapy with monoclonal antibodies by elimination of T-cell subsets in vivo. Nature 312 (5994):548-51. PMID: 6150440.Hale G, Clark M, Waldmann H. 1985 Therapeutic potential of rat monoclonal antibodies: isotype specificity of antibody-dependent cell-mediated cytotoxicity with human lymphocytes. J Immunol. 134(5):3056-61 PMID: 3980990. Cobbold S, Martin G, Waldmann H. 1986 Monoclonal antibodies for the prevention of graft-versus-host disease and marrow graft rejection. The depletion of T cell subsets in vitro and in vivo. Transplantation. 42(3):239-47 PMID: 3529524. Cobbold S, Waldmann H. 1986 Skin allograft rejection by L3/T4+ and Lyt-2+ T cell subsets. Transplantation. 41(5):634-9. Erratum in: Transplantation 1986 42(3):332. PMID: 3085299.

Hyperlink to ECACC Cell Line Data Sheet: 87072285