YW 62.3.20
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Description
Description: Rat hybridoma producing antibody against mouse CD45 pan isoform reactive. Cambridge Enterprise Reference number AB62:www.enterprise.cam.ac.uk.
Also Known As:
Species: Rat
Growth Medium: Iscoves Modified Dulbeccos Medium (IMDM) + 2mM glutamine + 5% Foetal Bovine Serum (FBS). Where the cells are grown under static conditions, 0.02% EDTA, trypsin or mechanical agitation maybe required to dislodge them from the plastic.
Subculturing Procedure: Maintain cultures between 3-9 x 100,000 cells/ml. 5% CO2; 37°C. Saturation density 9 x 100,000/ml
Release Conditions: Yes
Depositor: Cambridge Enterprise Limited, Hauser Forum, 3 Charles Babbage Road, Cambridge CB3 0GT. Originators: Professor H Waldmann and Dr SP Cobbold when at the Department of Pathology, University of Cambridge
Originator: Yes
References: Watt SM, Gilmore DJ, Davis JM, Clark MR, Waldmann H 1987 Cell-surface markers on haemopoietic precursors. Reagents for the isolation and analysis of progenitor cell subpopulations. Mol Cell Probes. 1(4):297-326 PMID: 3330997.
Hyperlink to ECACC Cell Line Data Sheet: 11072202
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