ZR-75-1
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Description
Description: Derived from a malignant ascitic effusion in a 63 year old female Caucasian with infiltrating ductal carcinoma. The cells are reported to express both the wildtype and variant oestrogen receptors, progesterone receptor and other steroid hormones.
Also Known As:
Species: Human
Tissue: breast
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: RPMI 1640 + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3-5×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells grow very slowly, do not form a confluent monolayer and attach only lightly to the substrate. After subculture, it can take up to 7 days before cells re-attach. Detachment of cells can occur in PBS and trypsinisation can produce clumps of cells. To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. When subculturing, ensure the trypsin is completely removed by centrifuging the cells and resuspending them in fresh medium.
Release Conditions: No
Depositor:
Originator: No
References: Cancer Res 1978;38:3352; Cancer Res 1978;38:4327; Cancer Res 1993;53:5882
Hyperlink to ECACC Cell Line Data Sheet: 87012601
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